What is the catalog number for NFKB1 ELISA Kit (Rat)?

The catalog number for NFKB1 ELISA Kit (Rat) is OKEH01302-96W.

What sizes are available for NFKB1 ELISA Kit (Rat)?

NFKB1 ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is NFKB1 ELISA Kit (Rat) in stock?

NFKB1 ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

NFKB1 ELISA Kit (Rat)

Selected Variant

OKEH01302-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Gene Aliases

DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1.

Gene ID

81736

Accession Number

NP_001263640.1

Reactivity

Rat, Rattus norvegicus

Target

Nuclear factor NF-kappa-B p105 subunit

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NFKB1 ELISA Kit (Rat) (OKEH01302) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NFKB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NFKB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NFKB1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%

Sensitivity

0.092 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

57 kDa

Specificity

Natural and recombinant Rat Nuclear factor NF-kappa-B p105 subunit

Protein Length

522

NCBI Gene Symbol

NFKB1

Protein Name

Q63369

Gene Name URL

NFKB1

Nucleotide Accession Number

NM_001276711.1

Price

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