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TGM2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Transglutaminase 2

Gene Aliases

C polypeptide, protein-glutamine-gamma-glutamyltransferase; erythrocyte transglutaminase; G (h) ; heart G alpha (h) ; hhG alpha (h) ; hTG2; isopeptidase TGM2; protein G alpha (h) ; protein-glutamine deamidase TGM2; protein-glutamine dopaminyltransferase TGM2; protein-glutamine gamma-glutamyltransferase 2; protein-glutamine histaminyltransferase TGM2; protein-glutamine noradrenalinyltransferase TGM2; protein-glutamine serotonyltransferase TGM2; TG (C) ; TGase C; TGase H; TGase II; TGase-2; TGC; tissue transglutaminase; transglutaminase C; transglutaminase H; transglutaminase II; Transglutaminase-2; tTG; tTgase.

Gene ID

7052

Accession Number

NP_004604.2

Reactivity

Homo sapiens, Human

Target

Protein-glutamine gamma-glutamyltransferase 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TGM2 ELISA Kit (Human) (OKEH01258) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TGM2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TGM2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TGM2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

0.082 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

77 kDa

Notes

Formerly GWB-R2SD9X

Specificity

Natural and recombinant Human Protein-glutamine gamma-glutamyltransferase 2

Protein Length

687

NCBI Gene Symbol

TGM2

Protein Name

P21980

Gene Name URL

TGM2

Nucleotide Accession Number

NM_004613.2

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