PRDX6 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Peroxiredoxin 6
Gene Aliases
1-Cys;1-Cys peroxiredoxin;1-Cys PRX;24 kDa protein; acidic calcium-independent phospholipase A2; aiPLA2; antioxidant protein 2; AOP2; epididymis secretory sperm binding protein Li 128m; glutathione-dependent peroxiredoxin; HEL-S-128m; liver 2D page spot 40; LPC acyltransferase 5; LPCAT-5; lyso-PC acyltransferase 5; lysophosphatidylcholine acyltransferase 5; non-selenium glutathione peroxidase; NSGPx; p29; peroxiredoxin-6; PRX; red blood cells page spot 12.
Gene ID
9588
Accession Number
NP_004896.1
Reactivity
Homo sapiens, Human
Target
Peroxiredoxin-6
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PRDX6 ELISA Kit (Human) (OKEH01255) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRDX6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRDX6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRDX6 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
Sensitivity
4.6 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
25 kDa
Specificity
Natural and recombinant Human Peroxiredoxin-6
Protein Length
224
NCBI Gene Symbol
PRDX6
Protein Name
P30041
Gene Name URL
PRDX6
Nucleotide Accession Number
NM_004905.2
Frequently Asked Questions
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