What is the catalog number for HK3 ELISA Kit (Human)?

The catalog number for HK3 ELISA Kit (Human) is OKEH01253-96W.

What sizes are available for HK3 ELISA Kit (Human)?

HK3 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is HK3 ELISA Kit (Human) in stock?

HK3 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

HK3 ELISA Kit (Human)

Selected Variant

OKEH01253-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Hexokinase 3

Gene Aliases

ATP:D-hexose 6-phosphotransferase; hexokinase 3 (white cell) ; hexokinase type III; hexokinase-3; hexokinase-C; HK III; HKIII; HXK3.

Gene ID

3101

Accession Number

NP_002106.2

Reactivity

Homo sapiens, Human

Target

Hexokinase-3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HK3 ELISA Kit (Human) (OKEH01253) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HK3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HK3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HK3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%

Sensitivity

0.076 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

99 kDa

Specificity

Natural and recombinant Human Hexokinase-3

Protein Length

923

NCBI Gene Symbol

HK3

Protein Name

P52790

Gene Name URL

HK3

Nucleotide Accession Number

NM_002115.2

Price

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