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STAT5A ELISA Kit (Bovine)

Product Specifications

CAS Number

7732-18-5

Gene Name

Signal transducer and activator of transcription 5A

Gene Aliases

Mammary gland factor; signal transducer and activator of transcription 5A; signal transducer and activator of transcription factor 5A; STAT5A, Mammary Gland Factor; STAT5A/MGF; STATA5.

Gene ID

282375

Accession Number

NP_001012691.1

Reactivity

Bos taurus, Bovine

Target

Signal transducer and activator of transcription 5A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology STAT5A ELISA Kit (Bovine) (OKEH01246) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for STAT5A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for STAT5A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample STAT5A captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

1.56-100ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%

Sensitivity

0.35 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

90 kDa

Specificity

Natural and recombinant Bovine Signal transducer and activator of transcription 5A

Protein Length

794

NCBI Gene Symbol

STAT5A

Protein Name

Q95115

Gene Name URL

STAT5A

Nucleotide Accession Number

NM_001012673.1

Frequently Asked Questions

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