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H2Q7 ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Histocompatibility 2, Q region locus 7

Gene Aliases

Class Ib MHC antigen Qa-2; H-2 class I histocompatibility antigen, Q7 alpha chain; H-2Q7; Pe; Ped; Q9; Qa; Qa-; Qa-2; QA-2 antigen; Qa7; Qa-7.

Gene ID

15018

Accession Number

NP_034524.3

Reactivity

Mouse, Mus musculus

Target

H-2 class I histocompatibility antigen, Q7 alpha chain

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology H2Q7 ELISA Kit (Mouse) (OKEH01222) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for H2Q7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for H2Q7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample H2Q7 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

7.8-500nmol/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%

Sensitivity

4.11nmol/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

38 kDa

Specificity

Natural and recombinant Mouse H-2 class I histocompatibility antigen, Q7 alpha chain

Protein Length

334

NCBI Gene Symbol

H2-Q7

Protein Name

P14429

Gene Name URL

H2-Q7

Nucleotide Accession Number

NM_010394.4

Frequently Asked Questions

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