What is the catalog number for LDHD ELISA Kit (Human)?

The catalog number for LDHD ELISA Kit (Human) is OKEH01221-96W.

What sizes are available for LDHD ELISA Kit (Human)?

LDHD ELISA Kit (Human) is available in the following size(s): 96 Well.

Is LDHD ELISA Kit (Human) in stock?

LDHD ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

LDHD ELISA Kit (Human)

Selected Variant

OKEH01221-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Lactate dehydrogenase D

Gene Aliases

DLACD; D-lactate dehydrogenase; DLD; probable D-lactate dehydrogenase, mitochondrial.

Gene ID

197257

Accession Number

NP_705690.2

Reactivity

Homo sapiens, Human

Target

Probable D-lactate dehydrogenase, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology LDHD ELISA Kit (Human) (OKEH01221) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LDHD has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LDHD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LDHD captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

0.34 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

55 kDa

Specificity

Natural and recombinant Human Probable D-lactate dehydrogenase, mitochondrial

Protein Length

507

NCBI Gene Symbol

LDHD

Protein Name

Q86WU2

Gene Name URL

LDHD

Nucleotide Accession Number

NM_153486.3

Price

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