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GNRHR ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Gonadotropin releasing hormone receptor

Gene Aliases

GnRH receptor; gnRH-R; GNRHR1; gonadotropin-releasing hormone (type 1) receptor 1; gonadotropin-releasing hormone receptor; GRHR; HH7; leutinizing hormone releasing horomone receptor; leutinizing-releasing hormone receptor; LHRHR; LRHR; luliberin receptor; type I GnRH receptor.

Gene ID

2798

Accession Number

NP_000397.1

Reactivity

Homo sapiens, Human

Target

Gonadotropin-releasing hormone receptor

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology GNRHR ELISA Kit (Human) (OKEH01209) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GNRHR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GNRHR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GNRHR captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%

Sensitivity

0.076 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

38 kDa

Specificity

Natural and recombinant Human Gonadotropin-releasing hormone receptor

Protein Length

328

NCBI Gene Symbol

GNRHR

Protein Name

P30968

Gene Name URL

GNRHR

Nucleotide Accession Number

NM_000406.2

Frequently Asked Questions

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