GNRHR ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Gonadotropin releasing hormone receptor
Gene Aliases
GnRH receptor; gnRH-R; GNRHR1; gonadotropin-releasing hormone (type 1) receptor 1; gonadotropin-releasing hormone receptor; GRHR; HH7; leutinizing hormone releasing horomone receptor; leutinizing-releasing hormone receptor; LHRHR; LRHR; luliberin receptor; type I GnRH receptor.
Gene ID
2798
Accession Number
NP_000397.1
Reactivity
Homo sapiens, Human
Target
Gonadotropin-releasing hormone receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GNRHR ELISA Kit (Human) (OKEH01209) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GNRHR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GNRHR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GNRHR captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
Sensitivity
0.076 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
38 kDa
Specificity
Natural and recombinant Human Gonadotropin-releasing hormone receptor
Protein Length
328
NCBI Gene Symbol
GNRHR
Protein Name
P30968
Gene Name URL
GNRHR
Nucleotide Accession Number
NM_000406.2
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