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PRKAB1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Protein kinase AMP-activated non-catalytic subunit beta 1

Gene Aliases

5'-AMP-activated protein kinase beta-1 subunit;5'-AMP-activated protein kinase subunit beta-1; AMP-activated protein kinase beta subunit; AMPK; AMPK beta 1; AMPK beta -1 chain; AMPK subunit beta-1; AMPKb; HAMPKb; protein kinase, AMP-activated, beta 1 non-catalytic subunit; protein kinase, AMP-activated, noncatalytic, beta-1.

Gene ID

5564

Accession Number

NP_006244.2

Reactivity

Homo sapiens, Human

Target

5'-AMP-activated protein kinase subunit beta-1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PRKAB1 ELISA Kit (Human) (OKEH01208) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRKAB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRKAB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRKAB1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%

Sensitivity

0.16 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

30 kDa

Specificity

Natural and recombinant Human 5'-AMP-activated protein kinase subunit beta-1

Protein Length

270

NCBI Gene Symbol

PRKAB1

Protein Name

Q9Y478

Gene Name URL

PRKAB1

Nucleotide Accession Number

NM_006253.4

Frequently Asked Questions

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