PLA2G4A ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Phospholipase A2 group IVA
Gene Aliases
Calcium-dependent phospholipid-binding protein; cPLA2; cPLA2-alpha; cytosolic phospholipase A2; GURDP; lysophospholipase; phosphatidylcholine 2-acylhydrolase; Phospholipase A2 group IVA; phospholipase A2, group IVA (cytosolic, calcium-dependent) ; PLA2G4.
Gene ID
5321
Accession Number
NP_001298122.1
Reactivity
Homo sapiens, Human
Target
Cytosolic phospholipase A2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PLA2G4A ELISA Kit (Human) (OKEH01172) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PLA2G4A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PLA2G4A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PLA2G4A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
12.4 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
85 kDa
Specificity
Natural and recombinant Human Cytosolic phospholipase A2
Protein Length
749
NCBI Gene Symbol
PLA2G4A
Protein Name
P47712
Gene Name URL
PLA2G4A
Nucleotide Accession Number
NM_001311193.1
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