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CTSA ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Cathepsin A

Gene Aliases

Beta-galactosidase 2; beta-galactosidase protective protein; carboxypeptidase C; Carboxypeptidase L; carboxypeptidase Y-like kininase; carboxypeptidase-L; Cathepsin A; deamidase; GLB2; GSL; lysosomal carboxypeptidase A; lysosomal protective protein; NGBE; PPCA; PPGB; protective protein cathepsin A; Protective protein for beta-galactosidase; urinary kininase.

Gene ID

5476

Accession Number

NP_000299.2

Reactivity

Homo sapiens, Human

Target

Lysosomal protective protein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CTSA ELISA Kit (Human) (OKEH01165) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CTSA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CTSA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CTSA captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%

Sensitivity

33 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

54 kDa

Specificity

Natural and recombinant Human Lysosomal protective protein

Protein Length

480

NCBI Gene Symbol

CTSA

Protein Name

P10619

Gene Name URL

CTSA

Nucleotide Accession Number

NM_000308.3

Frequently Asked Questions

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