PTMA ELISA Kit (Human)
Product Specifications
Gene Name
Prothymosin alpha
Gene Aliases
Gene sequence 28; prothymosin alpha; prothymosin alpha protein; prothymosin-I+/-; TMSA.
Gene ID
5757
Accession Number
NP_001092755.1
Reactivity
Homo sapiens, Human
Target
Prothymosin alpha
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology PTMA ELISA Kit (Human) (OKEH01154) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human PTMA antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PTMA and incubated. The PTMA found in the sample or standards competes with the biotinylated PTMA for limited binding sites on the immobilized anti-human PTMA antibody. Excess unbound biotinylated PTMA and sample or standard PTMA is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PTMA captured in the well and inversely proportional to the amount of PTMA which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Sensitivity
0.039 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
12 kDa
Specificity
Natural and recombinant Human Prothymosin alpha
Protein Length
111
NCBI Gene Symbol
PTMA
Protein Name
P06454
Gene Name URL
PTMA
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001099285.1
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