FCGR3A ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Fc fragment of IgG receptor IIIa
Gene Aliases
CD16; CD16A; CD16a antigen; Fc fragment of IgG, low affinity III, receptor for (CD16) ; Fc fragment of IgG, low affinity IIIa, receptor (CD16a) ; Fc gamma receptor III-A; FCG3; Fc-gamma receptor III-2 (CD 16) ; Fc-gamma receptor IIIb (CD16) ; Fc-gamma RIII-alpha; FcgammaRIIIA; FCGR3; FCGRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; igG Fc receptor III-2; IMD20; immunoglobulin G Fc receptor III; low affinity immunoglobulin gamma Fc region receptor III-A; low affinity immunoglobulin gamma receptor III-a Fc fragment; neutrophil-specific antigen NA.
Gene ID
2214
Accession Number
NP_000560.5
Reactivity
Homo sapiens, Human
Target
Low affinity immunoglobulin gamma Fc region receptor III-A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology FCGR3A ELISA Kit (Human) (OKEH01135) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FCGR3A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FCGR3A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FCGR3A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
Sensitivity
0.12 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
29 kDa
Specificity
Natural and recombinant Human Low affinity immunoglobulin gamma Fc region receptor III-A
Protein Length
254
NCBI Gene Symbol
FCGR3A
Protein Name
P08637
Gene Name URL
FCGR3A
Nucleotide Accession Number
NM_000569.6
Frequently Asked Questions
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