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FCGR3A ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Fc fragment of IgG receptor IIIa

Gene Aliases

CD16; CD16A; CD16a antigen; Fc fragment of IgG, low affinity III, receptor for (CD16) ; Fc fragment of IgG, low affinity IIIa, receptor (CD16a) ; Fc gamma receptor III-A; FCG3; Fc-gamma receptor III-2 (CD 16) ; Fc-gamma receptor IIIb (CD16) ; Fc-gamma RIII-alpha; FcgammaRIIIA; FCGR3; FCGRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; igG Fc receptor III-2; IMD20; immunoglobulin G Fc receptor III; low affinity immunoglobulin gamma Fc region receptor III-A; low affinity immunoglobulin gamma receptor III-a Fc fragment; neutrophil-specific antigen NA.

Gene ID

2214

Accession Number

NP_000560.5

Reactivity

Homo sapiens, Human

Target

Low affinity immunoglobulin gamma Fc region receptor III-A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FCGR3A ELISA Kit (Human) (OKEH01135) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FCGR3A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FCGR3A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FCGR3A captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%

Sensitivity

0.12 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

29 kDa

Specificity

Natural and recombinant Human Low affinity immunoglobulin gamma Fc region receptor III-A

Protein Length

254

NCBI Gene Symbol

FCGR3A

Protein Name

P08637

Gene Name URL

FCGR3A

Nucleotide Accession Number

NM_000569.6

Frequently Asked Questions

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