What is the catalog number for RPTOR ELISA Kit (Human)?

The catalog number for RPTOR ELISA Kit (Human) is OKEH01117-96W.

What sizes are available for RPTOR ELISA Kit (Human)?

RPTOR ELISA Kit (Human) is available in the following size(s): 96 Well.

Is RPTOR ELISA Kit (Human) in stock?

RPTOR ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

RPTOR ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Regulatory associated protein of MTOR complex 1

Gene Aliases

KOG1; Mip1; p150 target of rapamycin (TOR) -scaffold protein; p150 target of rapamycin (TOR) -scaffold protein containing WD-repeats; raptor; regulatory-associated protein of mTOR.

Gene ID

57521

Accession Number

NP_001156506.1

Reactivity

Homo sapiens, Human

Target

Regulatory-associated protein of mTOR

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology RPTOR ELISA Kit (Human) (OKEH01117) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RPTOR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RPTOR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RPTOR captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol