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HSPA5 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Heat shock protein family A (Hsp70) member 5

Gene Aliases

78 kDa glucose-regulated protein; binding-immunoglobulin protein; BIP; endoplasmic reticulum chaperone BiP; GRP 78; GRP78; GRP-78; heat shock 70 kDa protein 5; heat shock 70kD protein 5; heat shock 70kDa protein 5 (glucose-regulated protein) ; heat shock protein 5; heat shock protein 70 family protein 5; heat shock protein family A member 5; HSP70 family protein 5; immunoglobulin heavy chain-binding protein; steroidogenesis-activator polypeptide.

Gene ID

25617

Accession Number

NP_037215.1

Reactivity

Rat, Rattus norvegicus

Target

Endoplasmic reticulum chaperone BiP

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HSPA5 ELISA Kit (Rat) (OKEH01108) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPA5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSPA5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPA5 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81%

Sensitivity

0.17 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

72 kDa

Specificity

Natural and recombinant Rat 78 kDa glucose-regulated protein

Protein Length

654

NCBI Gene Symbol

Hspa5

Protein Name

P06761

Gene Name URL

Hspa5

Nucleotide Accession Number

NM_013083.2

Frequently Asked Questions

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