What is the catalog number for IRAK1 ELISA Kit (Human)?

The catalog number for IRAK1 ELISA Kit (Human) is OKEH01082-96W.

What sizes are available for IRAK1 ELISA Kit (Human)?

IRAK1 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is IRAK1 ELISA Kit (Human) in stock?

IRAK1 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

IRAK1 ELISA Kit (Human)

Selected Variant

OKEH01082-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Interleukin 1 receptor associated kinase 1

Gene Aliases

Interleukin-1 receptor-associated kinase 1; IRAK; IRAK-1; pelle; Pelle homolog.

Gene ID

3654

Accession Number

NP_001020413.1

Reactivity

Homo sapiens, Human

Target

Interleukin-1 receptor-associated kinase 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology IRAK1 ELISA Kit (Human) (OKEH01082) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IRAK1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IRAK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IRAK1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

77 kDa

Specificity

Natural and recombinant Human Interleukin-1 receptor-associated kinase 1

Protein Length

712

NCBI Gene Symbol

IRAK1

Protein Name

P51617

Gene Name URL

IRAK1

Nucleotide Accession Number

NM_001025242.1

Price

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