TXNRD1 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Thioredoxin reductase 1
Gene Aliases
Gene associated with retinoic and IFN-induced mortality 12 protein; gene associated with retinoic and interferon-induced mortality 12 protein; GRIM-12; KM-102-derived reductase-like factor; oxidoreductase; selenoprotein TXNRD1; testis tissue sperm-binding protein Li 46a; thioredoxin reductase 1, cytoplasmic; thioredoxin reductase GRIM-12; thioredoxin reductase TR1; TR; TR1; TRXR1; TXNR.
Gene ID
7296
Accession Number
NP_001087240.1
Reactivity
Homo sapiens, Human
Target
Thioredoxin reductase 1, cytoplasmic
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TXNRD1 ELISA Kit (Human) (OKEH01074) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TXNRD1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TXNRD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TXNRD1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
71 kDa
Specificity
Natural and recombinant Human Thioredoxin reductase 1, cytoplasmic
Protein Length
649
NCBI Gene Symbol
TXNRD1
Protein Name
Q16881
Gene Name URL
TXNRD1
Nucleotide Accession Number
NM_001093771.2
Frequently Asked Questions
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