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S100A12 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

S100 calcium binding protein A12

Gene Aliases

CAAF1; CAGC; calcitermin; calcium-binding protein in amniotic fluid 1; calgranulin C; Calgranulin-C; CGRP; ENRAGE; EN-RAGE; extracellular newly identified RAGE-binding protein; migration inhibitory factor-related protein 6; MRP6; MRP-6; neutrophil S100 protein; p6; protein S100-A12; S100 calcium-binding protein A12.

Gene ID

6283

Accession Number

NP_005612.1

Reactivity

Homo sapiens, Human

Target

Protein S100-A12

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology S100A12 ELISA Kit (Human) (OKEH01072) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S100A12 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for S100A12 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S100A12 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%

Sensitivity

7.8 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

11 kDa

Specificity

Natural and recombinant Human Protein S100-A12

Protein Length

92

NCBI Gene Symbol

S100A12

Protein Name

P80511

Gene Name URL

S100A12

Nucleotide Accession Number

NM_005621.1

Frequently Asked Questions

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