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SORD ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Sorbitol dehydrogenase

Gene Aliases

(R, R) -butanediol dehydrogenase; epididymis secretory sperm binding protein Li 95n; HEL-S-95n; L-iditol 2-dehydrogenase; polyol dehydrogenase; RDH; ribitol dehydrogenase; SDH; sorbitol dehydrogenase; SORD1; SORDD; XDH; xylitol dehydrogenase.

Gene ID

6652

Accession Number

NP_003095.2

Reactivity

Homo sapiens, Human

Target

Sorbitol dehydrogenase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SORD ELISA Kit (Human) (OKEH01065) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human SORD antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SORD and incubated. The SORD found in the sample or standards competes with the biotinylated SORD for limited binding sites on the immobilized anti-human SORD antibody. Excess unbound biotinylated SORD and sample or standard SORD is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SORD captured in the well and inversely proportional to the amount of SORD which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.312-20U/L

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

0.2U/L

Reconstitution

Store as indicated in product manual.

Molecular Weight

38 kDa

Specificity

Natural and recombinant Human Sorbitol dehydrogenase

Protein Length

357

NCBI Gene Symbol

SORD

Protein Name

Q00796

Gene Name URL

SORD

Nucleotide Accession Number

NM_003104.5

Frequently Asked Questions

More Discoveries

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