SORD ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Sorbitol dehydrogenase
Gene Aliases
(R, R) -butanediol dehydrogenase; epididymis secretory sperm binding protein Li 95n; HEL-S-95n; L-iditol 2-dehydrogenase; polyol dehydrogenase; RDH; ribitol dehydrogenase; SDH; sorbitol dehydrogenase; SORD1; SORDD; XDH; xylitol dehydrogenase.
Gene ID
6652
Accession Number
NP_003095.2
Reactivity
Homo sapiens, Human
Target
Sorbitol dehydrogenase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SORD ELISA Kit (Human) (OKEH01065) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human SORD antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SORD and incubated. The SORD found in the sample or standards competes with the biotinylated SORD for limited binding sites on the immobilized anti-human SORD antibody. Excess unbound biotinylated SORD and sample or standard SORD is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SORD captured in the well and inversely proportional to the amount of SORD which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20U/L
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
0.2U/L
Reconstitution
Store as indicated in product manual.
Molecular Weight
38 kDa
Specificity
Natural and recombinant Human Sorbitol dehydrogenase
Protein Length
357
NCBI Gene Symbol
SORD
Protein Name
Q00796
Gene Name URL
SORD
Nucleotide Accession Number
NM_003104.5
Frequently Asked Questions
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