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NOX4 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

NADPH oxidase 4

Gene Aliases

Kidney oxidase-1; kidney superoxide-producing NADPH oxidase; kox-1; NADPH oxidase 4.

Gene ID

85431

Accession Number

NP_445976.1

Reactivity

Rat, Rattus norvegicus

Target

NADPH oxidase 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NOX4 ELISA Kit (Rat) (OKEH01057) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NOX4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NOX4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NOX4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%

Sensitivity

0.16 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

66 kDa

Specificity

Natural and recombinant Rat NADPH oxidase 4

Protein Length

578

NCBI Gene Symbol

Nox4

Protein Name

Q924V1

Gene Name URL

Nox4

Nucleotide Accession Number

NM_053524.1

Frequently Asked Questions

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