What is the catalog number for COL27A1 ELISA Kit (Human)?

The catalog number for COL27A1 ELISA Kit (Human) is OKEH01036-96W.

What sizes are available for COL27A1 ELISA Kit (Human)?

COL27A1 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is COL27A1 ELISA Kit (Human) in stock?

COL27A1 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

COL27A1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Collagen type XXVII alpha 1 chain

Gene Aliases

Collagen alpha-1 (XXVII) chain; collagen, type XXVII, alpha 1; STLS.

Gene ID

85301

Accession Number

NP_116277.2

Reactivity

Homo sapiens, Human

Target

Collagen alpha-1 (XXVII) chain

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology COL27A1 ELISA Kit (Human) (OKEH01036) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human COL27A1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated COL27A1 and incubated. The COL27A1 found in the sample or standards competes with the biotinylated COL27A1 for limited binding sites on the immobilized anti-human COL27A1 antibody. Excess unbound biotinylated COL27A1 and sample or standard COL27A1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated COL27A1 captured in the well and inversely proportional to the amount of COL27A1 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol