PNLIP ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Pancreatic lipase
Gene Aliases
Pancreatic triacylglycerol lipase; PL; PNLIPD; PTL; triacylglycerol acylhydrolase; triacylglycerol lipase.
Gene ID
5406
Accession Number
NP_000927.1
Reactivity
Homo sapiens, Human
Target
Pancreatic triacylglycerol lipase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PNLIP ELISA Kit (Human) (OKEH01028) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human PNLIP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PNLIP and incubated. The PNLIP found in the sample or standards competes with the biotinylated PNLIP for limited binding sites on the immobilized anti-human PNLIP antibody. Excess unbound biotinylated PNLIP and sample or standard PNLIP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PNLIP captured in the well and inversely proportional to the amount of PNLIP which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
Sensitivity
1.17U/L
Reconstitution
Store as indicated in product manual.
Molecular Weight
51 kDa
Specificity
Natural and recombinant Human Pancreatic triacylglycerol lipase
Protein Length
465
NCBI Gene Symbol
PNLIP
Protein Name
P16233
Gene Name URL
PNLIP
Nucleotide Accession Number
NM_000936.2
Frequently Asked Questions
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