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PNLIP ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Pancreatic lipase

Gene Aliases

Pancreatic triacylglycerol lipase; PL; PNLIPD; PTL; triacylglycerol acylhydrolase; triacylglycerol lipase.

Gene ID

5406

Accession Number

NP_000927.1

Reactivity

Homo sapiens, Human

Target

Pancreatic triacylglycerol lipase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PNLIP ELISA Kit (Human) (OKEH01028) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human PNLIP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PNLIP and incubated. The PNLIP found in the sample or standards competes with the biotinylated PNLIP for limited binding sites on the immobilized anti-human PNLIP antibody. Excess unbound biotinylated PNLIP and sample or standard PNLIP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PNLIP captured in the well and inversely proportional to the amount of PNLIP which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%

Sensitivity

1.17U/L

Reconstitution

Store as indicated in product manual.

Molecular Weight

51 kDa

Specificity

Natural and recombinant Human Pancreatic triacylglycerol lipase

Protein Length

465

NCBI Gene Symbol

PNLIP

Protein Name

P16233

Gene Name URL

PNLIP

Nucleotide Accession Number

NM_000936.2

Frequently Asked Questions

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