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EFNA1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Ephrin A1

Gene Aliases

B61; ECKLG; EFL1; eph-related receptor tyrosine kinase ligand 1; ephrin-A1; epididymis secretory sperm binding protein; EPLG1; gastric cancer metastasis associated long noncoding RNA; GMAN; immediate early response protein B61; LERK1; LERK-1; ligand of eph-related kinase 1; TNF alpha-induced protein 4; TNFAIP4; Tumor necrosis factor alpha-induced protein 4; tumor necrosis factor, alpha-induced protein 4.

Gene ID

1942

Accession Number

NP_004419.2

Reactivity

Homo sapiens, Human

Target

Ephrin-A1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology EFNA1 ELISA Kit (Human) (OKEH01001) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EFNA1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EFNA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EFNA1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%

Sensitivity

0.098 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

24 kDa

Specificity

Natural and recombinant Human Ephrin-A1

Protein Length

205

NCBI Gene Symbol

EFNA1

Protein Name

P20827

Gene Name URL

EFNA1

Nucleotide Accession Number

NM_004428.2

Frequently Asked Questions

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