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PPP3R1 ELISA Kit (Rat)

Selected Variant

OKEH00948-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Protein phosphatase 3, regulatory subunit B, alpha

Gene Aliases

Calcineurin subunit B type 1; protein phosphatase 2B regulatory subunit 1; Protein phosphatase 3 regulatory subunit B alpha isoform 1; protein phosphatase 3, regulatory subunit B, alpha isoform.

Gene ID

29748

Accession Number

NP_059005.1

Reactivity

Rat, Rattus norvegicus

Target

Calcineurin subunit B type 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PPP3R1 ELISA Kit (Rat) (OKEH00948) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PPP3R1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PPP3R1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PPP3R1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%

Sensitivity

0.086 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

19 kDa

Specificity

Natural and recombinant Rat Calcineurin subunit B type 1

Protein Length

170

NCBI Gene Symbol

Ppp3r1

Protein Name

P63100

Gene Name URL

Ppp3r1

Nucleotide Accession Number

NM_017309.2

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