Got1 ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Glutamic-oxaloacetic transaminase 1, soluble
Gene Aliases
AI789014; aspartate aminotransferase, cytoplasmic; cA; cAspAT; cCAT; cysteine aminotransferase, cytoplasmic; cysteine transaminase, cytoplasmic; cytosolic aspartate aminotransferase; Glutamate oxaloacetate transaminase 1; glutamate oxaloacetate transaminase 1, soluble; Got-; Got-1; transaminase A.
Gene ID
14718
Accession Number
NP_034454.2
Reactivity
Mouse, Mus musculus
Target
Aspartate aminotransferase, cytoplasmic
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Got1 ELISA Kit (Mouse) (OKEH00877) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Got1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Got1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Got1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
1.56-100U/L
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%
Sensitivity
0.88U/L
Reconstitution
Store as indicated in product manual.
Molecular Weight
46 kDa
Specificity
Natural and recombinant Mouse Aspartate aminotransferase, cytoplasmic
Protein Length
413
NCBI Gene Symbol
Got1
Protein Name
P05201
Gene Name URL
Got1
Nucleotide Accession Number
NM_010324.2
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