GPER1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

G protein-coupled estrogen receptor 1

Gene Aliases

CEPR; chemoattractant receptor-like 2; chemokine receptor-like 2; CMKRL2; constitutively expressed peptide-like receptor; DRY12; FEG-1; flow-induced endothelial G-protein coupled receptor 1; G protein-coupled estrogen receptor 1; G protein-coupled receptor 30; GPCR-Br; GPER; GPR30; G-protein coupled estrogen receptor 1; G-protein coupled receptor 30; heptahelix receptor; IL8-related receptor DRY12; LERGU; LERGU2; LyGPR; lymphocyte-derived G-protein coupled receptor; membrane estrogen receptor; mER.

Gene ID

2852

Accession Number

NP_001035055.1

Reactivity

Homo sapiens, Human

Target

G-protein coupled estrogen receptor 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology GPER1 ELISA Kit (Human) (OKEH00856) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GPER1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GPER1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GPER1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol