GPER1 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
G protein-coupled estrogen receptor 1
Gene Aliases
CEPR; chemoattractant receptor-like 2; chemokine receptor-like 2; CMKRL2; constitutively expressed peptide-like receptor; DRY12; FEG-1; flow-induced endothelial G-protein coupled receptor 1; G protein-coupled estrogen receptor 1; G protein-coupled receptor 30; GPCR-Br; GPER; GPR30; G-protein coupled estrogen receptor 1; G-protein coupled receptor 30; heptahelix receptor; IL8-related receptor DRY12; LERGU; LERGU2; LyGPR; lymphocyte-derived G-protein coupled receptor; membrane estrogen receptor; mER.
Gene ID
2852
Accession Number
NP_001035055.1
Reactivity
Homo sapiens, Human
Target
G-protein coupled estrogen receptor 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GPER1 ELISA Kit (Human) (OKEH00856) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GPER1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GPER1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GPER1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110%
Sensitivity
0.127 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
42 kDa
Notes
Formerly GWB-H4VOI7
Specificity
Natural and recombinant Human G-protein coupled estrogen receptor 1
Protein Length
375
NCBI Gene Symbol
GPER1
Protein Name
Q99527
Gene Name URL
GPER1
Nucleotide Accession Number
NM_001039966.1
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