What is the catalog number for ALPL ELISA Kit (Mouse)?

The catalog number for ALPL ELISA Kit (Mouse) is OKEH00758-96W.

What sizes are available for ALPL ELISA Kit (Mouse)?

ALPL ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is ALPL ELISA Kit (Mouse) in stock?

ALPL ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

ALPL ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Alkaline phosphatase, liver/bone/kidney

Gene Aliases

Ak; Akp; Akp2; Akp-2; Alkaline phosphatase 2; alkaline phosphatase 2, liver; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, tissue-nonspecific isozyme; ALP; APTNAP; T; TNAP; TNSALP.

Gene ID

11647

Accession Number

NP_001274101.1

Reactivity

Mouse, Mus musculus

Target

Alkaline phosphatase, tissue-nonspecific isozyme

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ALPL ELISA Kit (Mouse) (OKEH00758) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ALPL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ALPL and incubated. The ALPL found in the sample or standards competes with the biotinylated ALPL for limited binding sites on the immobilized anti-human ALPL antibody. Excess unbound biotinylated ALPL and sample or standard ALPL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ALPL captured in the well and inversely proportional to the amount of ALPL which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol