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TMEM158 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Transmembrane protein 158

Gene Aliases

40 kDa BINP-binding protein; BBP; BINP receptor; brain injury-derived neurotrophic peptide (BINP) binding protein; brain specific binding protein; p40BBP; Ras induced senescence 1; Ras-induced senescence protein 1; RIS1; transmembrane protein 158.

Gene ID

25907

Accession Number

NP_056259.2

Reactivity

Homo sapiens, Human

Target

Transmembrane protein 158

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TMEM158 ELISA Kit (Human) (OKEH00746) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TMEM158 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TMEM158 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TMEM158 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%

Sensitivity

0.045 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

30 kDa

Specificity

Natural and recombinant Human Transmembrane protein 158

Protein Length

300

NCBI Gene Symbol

TMEM158

Protein Name

Q8WZ71

Gene Name URL

TMEM158

Nucleotide Accession Number

NM_015444.2

Frequently Asked Questions

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