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SRGN ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Serglycin

Gene Aliases

Hematopoetic proteoglycan core peptide; hematopoetic proteoglycan core protein; hematopoietic proteoglycan core protein; p.PG; platelet proteoglycan core protein; PPG; PRG; PRG1; proteoglycan 1, secretory granule; proteoglycan protein core for mast cell secretory granule; secretory granule proteoglycan core peptide; secretory granule proteoglycan core protein; serglycin; serglycin proteoglycan.

Gene ID

5552

Accession Number

NP_002718.2

Reactivity

Homo sapiens, Human

Target

Serglycin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SRGN ELISA Kit (Human) (OKEH00741) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SRGN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SRGN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SRGN captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 1%

Sensitivity

0.086 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

18 kDa

Notes

Formerly GWB-H4VPLJ

Specificity

Natural and recombinant Human Serglycin

Protein Length

158

NCBI Gene Symbol

SRGN

Protein Name

P10124

Gene Name URL

SRGN

Nucleotide Accession Number

NM_002727.3

Frequently Asked Questions

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