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CAMP ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Cathelicidin antimicrobial peptide

Gene Aliases

18 kDa cationic antimicrobial protein; CAP18; CAP-18; cathelicidin antimicrobial peptide; CRAMP; epididymis secretory sperm binding protein; FALL39; FALL-39; HSD26; LL37.

Gene ID

820

Accession Number

NP_004336.3

Reactivity

Homo sapiens, Human

Target

Cathelicidin antimicrobial peptide

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CAMP ELISA Kit (Human) (OKEH00728) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for CAMP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAMP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CAMP captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

125-8000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81%

Sensitivity

44 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

19 kDa

Notes

Formerly GWB-AO5IR0

Specificity

Natural and recombinant Human Cathelicidin antimicrobial peptide

Protein Length

170

NCBI Gene Symbol

CAMP

Protein Name

P49913

Gene Name URL

CAMP

Nucleotide Accession Number

NM_004345.4

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