CAMP ELISA Kit (Human)
Product Specifications
Gene Name
Cathelicidin antimicrobial peptide
Gene Aliases
18 kDa cationic antimicrobial protein; CAP18; CAP-18; cathelicidin antimicrobial peptide; CRAMP; epididymis secretory sperm binding protein; FALL39; FALL-39; HSD26; LL37.
Gene ID
820
Accession Number
NP_004336.3
Reactivity
Homo sapiens, Human
Target
Cathelicidin antimicrobial peptide
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CAMP ELISA Kit (Human) (OKEH00728) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for CAMP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAMP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CAMP captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
125-8000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81%
Sensitivity
44 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
19 kDa
Notes
Formerly GWB-AO5IR0
Specificity
Natural and recombinant Human Cathelicidin antimicrobial peptide
Protein Length
170
NCBI Gene Symbol
CAMP
Protein Name
P49913
Gene Name URL
CAMP
CAS Number
7732-18-5
Nucleotide Accession Number
NM_004345.4
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