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HBG1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Hemoglobin subunit gamma 1

Gene Aliases

A-gamma globin; gamma A hemoglobin; gamma globin; gamma-1-globin; hb F Agamma; HBGA; HBGR; HBG-T2; hemoglobin gamma-1 chain; hemoglobin gamma-a chain; hemoglobin subunit gamma-1; hemoglobin, gamma A; hemoglobin, gamma, regulator of; HSGGL1; PRO2979.

Gene ID

3047

Accession Number

NP_000550.2

Reactivity

Homo sapiens, Human

Target

Hemoglobin subunit gamma-1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HBG1 ELISA Kit (Human) (OKEH00697) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HBG1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HBG1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HBG1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

6.25-400ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%

Sensitivity

3.1 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

16 kDa

Specificity

Natural and recombinant Human Hemoglobin subunit gamma-1

Protein Length

147

NCBI Gene Symbol

HBG1

Protein Name

P69891

Gene Name URL

HBG1

Nucleotide Accession Number

NM_000559.2

Frequently Asked Questions

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