What is the catalog number for PPIA ELISA Kit (Human)?

The catalog number for PPIA ELISA Kit (Human) is OKEH00687-96W.

What sizes are available for PPIA ELISA Kit (Human)?

PPIA ELISA Kit (Human) is available in the following size(s): 96 Well.

Is PPIA ELISA Kit (Human) in stock?

PPIA ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

PPIA ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Peptidylprolyl isomerase A

Gene Aliases

Cyclophilin A; cyclosporin A-binding protein; CYPA; CYPH; epididymis secretory sperm binding protein Li 69p; HEL-S-69p; peptidyl-prolyl cis-trans isomerase A; peptidylprolyl isomerase A (cyclophilin A) ; PPIase A; rotamase A; T cell cyclophilin.

Gene ID

5478

Accession Number

NP_001287910.1

Reactivity

Homo sapiens, Human

Target

Peptidyl-prolyl cis-trans isomerase A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PPIA ELISA Kit (Human) (OKEH00687) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human PPIA antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PPIA and incubated. The PPIA found in the sample or standards competes with the biotinylated PPIA for limited binding sites on the immobilized anti-human PPIA antibody. Excess unbound biotinylated PPIA and sample or standard PPIA is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PPIA captured in the well and inversely proportional to the amount of PPIA which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol