LAG3 ELISA Kit (Human)
Product Specifications
Gene Name
Lymphocyte activating 3
Gene Aliases
CD223; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Protein FDC.
Gene ID
3902
Accession Number
NP_002277.4
Reactivity
Homo sapiens, Human
Target
Lymphocyte activation gene 3 protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LAG3 ELISA Kit (Human) (OKEH00667) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LAG3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LAG3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LAG3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%
Sensitivity
45 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
57 kDa
Notes
Formerly GWB-1YFX16
Specificity
Natural and recombinant Human Lymphocyte activation gene 3 protein
Protein Length
525
NCBI Gene Symbol
LAG3
Protein Name
P18627
Gene Name URL
LAG3
CAS Number
7732-18-5
Nucleotide Accession Number
NM_002286.5
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