HSPB6 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Heat shock protein family B (small) member 6
Gene Aliases
Epididymis luminal protein 55; epididymis secretory sperm binding protein; heat shock 20 kDa-like protein p20; heat shock protein beta-6; heat shock protein family B (small) member B6; heat shock protein, alpha-crystallin-related, B6; HEL55; Hsp20; PPP1R91; protein phosphatase 1, regulatory subunit 91.
Gene ID
126393
Accession Number
NP_653218.1
Reactivity
Homo sapiens, Human
Target
Heat shock protein beta-6
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HSPB6 ELISA Kit (Human) (OKEH00644) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPB6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSPB6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPB6 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
17 kDa
Specificity
Natural and recombinant Human Heat shock protein beta-6
Protein Length
160
NCBI Gene Symbol
HSPB6
Protein Name
O14558
Gene Name URL
HSPB6
Nucleotide Accession Number
NM_144617.2
Frequently Asked Questions
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