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HSPB6 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Heat shock protein family B (small) member 6

Gene Aliases

Epididymis luminal protein 55; epididymis secretory sperm binding protein; heat shock 20 kDa-like protein p20; heat shock protein beta-6; heat shock protein family B (small) member B6; heat shock protein, alpha-crystallin-related, B6; HEL55; Hsp20; PPP1R91; protein phosphatase 1, regulatory subunit 91.

Gene ID

126393

Accession Number

NP_653218.1

Reactivity

Homo sapiens, Human

Target

Heat shock protein beta-6

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HSPB6 ELISA Kit (Human) (OKEH00644) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPB6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSPB6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPB6 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

17 kDa

Specificity

Natural and recombinant Human Heat shock protein beta-6

Protein Length

160

NCBI Gene Symbol

HSPB6

Protein Name

O14558

Gene Name URL

HSPB6

Nucleotide Accession Number

NM_144617.2

Frequently Asked Questions

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