PLA2G7 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Phospholipase A2 group VII
Gene Aliases
1-alkyl-2-acetylglycerophosphocholine esterase;2-acetyl-1-alkylglycerophosphocholine esterase; group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA (2) ; LDL-PLA2; lipoprotein-associated phospholipase A2; LP-PLA2; PAF 2-acylhydrolase; PAF acetylhydrolase; PAFAD; PAFAH; phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) ; platelet-activating factor acetylhydrolase.
Gene ID
7941
Accession Number
NP_001161829.1
Reactivity
Homo sapiens, Human
Target
Platelet-activating factor acetylhydrolase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PLA2G7 ELISA Kit (Human) (OKEH00643) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PLA2G7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PLA2G7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PLA2G7 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
93.75-6000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Sensitivity
23.4 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
50 kDa
Specificity
Natural and recombinant Human Platelet-activating factor acetylhydrolase
Protein Length
441
NCBI Gene Symbol
PLA2G7
Protein Name
Q13093
Gene Name URL
PLA2G7
Nucleotide Accession Number
NM_001168357.1
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
MBS7067650 Inquire
Recombinant Salmonella paratyphi A Protease HtpX (htpX), partial
Ask
View Details MBS262912-01 48 Well
Chicken Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ELISA kit
Ask
View Details