What is the catalog number for PRL ELISA Kit (Chicken)?

The catalog number for PRL ELISA Kit (Chicken) is OKEH00637-96W.

What sizes are available for PRL ELISA Kit (Chicken)?

PRL ELISA Kit (Chicken) is available in the following size(s): 96 Well.

Is PRL ELISA Kit (Chicken) in stock?

PRL ELISA Kit (Chicken) is currently made to order / not held in immediate stock. Contact us for lead time.

PRL ELISA Kit (Chicken)

Product Specifications

CAS Number

7732-18-5

Gene Name

Prolactin

Gene Aliases

Prolactin; prolactin type 2a; prolactin type 2b; prolactin type 2c; prolactin type 3.

Gene ID

396453

Accession Number

NP_990797.2

Reactivity

Chicken, Gallus gallus

Target

Prolactin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology PRL ELISA Kit (Chicken) (OKEH00637) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Chicken PRL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PRL and incubated. The PRL found in the sample or standards competes with the biotinylated PRL for limited binding sites on the immobilized anti-Chicken PRL antibody. Excess unbound biotinylated PRL and sample or standard PRL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PRL captured in the well and inversely proportional to the amount of PRL which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol