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FSHB ELISA Kit (Salmon)

Product Specifications

CAS Number

7732-18-5

Gene Name

Follicle stimulating hormone beta subunit

Gene Aliases

HH24

Gene ID

2488

Accession Number

NP_001018090

Reactivity

Salmon

Target

Stimulates development of follicle and spermatogenesis in the reproductive organs.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FSHB ELISA Kit (Salmon) (OKEH00620) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FSHB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FSHB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FSHB captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10mIU/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%

Sensitivity

0.057mIU/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Salmon Follitropin subunit beta

NCBI Gene Symbol

FSHB

Protein Name

Follitropin subunit beta

Gene Name URL

FSHB

Nucleotide Accession Number

NM_001018080

Frequently Asked Questions

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