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GLP1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Zinc finger, GATA-like protein 1

Gene Aliases

GLP1, GLP-1, GATAD3

Gene ID

100125288

Accession Number

NP_001096637

Reactivity

Human

Target

Transcriptional repressor that plays a central role in somatic cells of the gonad and is required for germ cell development. Able to repress GATA transcription factor function

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology GLP1 ELISA Kit (Human) (OKEH00597) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GLP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GLP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GLP1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

15.6-1000pg/mL

Recovery

<= 5.9%

Sensitivity

8.2 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Glucagon-like peptide 1

NCBI Gene Symbol

GLP1

Protein Name

GATA-type zinc finger protein 1

Gene Name URL

GLP1

Nucleotide Accession Number

NM_001103167

Frequently Asked Questions

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