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MAGEC1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

MAGE family member C1

Gene Aliases

Cancer/testis antigen 7.1; cancer/testis antigen family 7, member 1; CT7; CT7.1; MAGE-C1 antigen; melanoma antigen family C, 1; melanoma antigen family C1; melanoma-associated antigen C1.

Gene ID

9947

Accession Number

NP_005453.2

Reactivity

Homo sapiens, Human

Target

Melanoma-associated antigen C1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MAGEC1 ELISA Kit (Human) (OKEH00582) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human MAGEC1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human MAGEC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human MAGEC1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%

Sensitivity

0.065 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

124 kDa

Specificity

Natural and recombinant Human Melanoma-associated antigen C1

Protein Length

1142

NCBI Gene Symbol

MAGEC1

Protein Name

O60732

Gene Name URL

MAGEC1

Nucleotide Accession Number

NM_005462.4

Frequently Asked Questions

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