MAGEC1 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
MAGE family member C1
Gene Aliases
Cancer/testis antigen 7.1; cancer/testis antigen family 7, member 1; CT7; CT7.1; MAGE-C1 antigen; melanoma antigen family C, 1; melanoma antigen family C1; melanoma-associated antigen C1.
Gene ID
9947
Accession Number
NP_005453.2
Reactivity
Homo sapiens, Human
Target
Melanoma-associated antigen C1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MAGEC1 ELISA Kit (Human) (OKEH00582) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human MAGEC1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human MAGEC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human MAGEC1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
Sensitivity
0.065 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
124 kDa
Specificity
Natural and recombinant Human Melanoma-associated antigen C1
Protein Length
1142
NCBI Gene Symbol
MAGEC1
Protein Name
O60732
Gene Name URL
MAGEC1
Nucleotide Accession Number
NM_005462.4
Frequently Asked Questions
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