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Aldh1a7 ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Aldehyde dehydrogenase family 1, subfamily A7

Gene Aliases

Ahd2-l; Ahd2-like; AI987940; Aldehyde dehydrogenase 1 (phenobarbitol inducible) ; aldehyde dehydrogenase family 1 member A7; aldehyde dehydrogenase family 1, subfamily A4; aldehyde dehydrogenase phenobarbital-inducible; aldehyde dehydrogenase, cytosolic 1; Aldh; ALDH class 1; Aldh1a4; ALDH-E1; Aldhpb; Aldh-pb; ALHDII.

Gene ID

26358

Accession Number

NP_036051.1

Reactivity

Mouse, Mus musculus

Target

Aldehyde dehydrogenase, cytosolic 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Aldh1a7 ELISA Kit (Mouse) (OKEH00581) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mouse Aldh1a7 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Mouse Aldh1a7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mouse Aldh1a7 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%

Sensitivity

40.5 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

55 kDa

Specificity

Natural and recombinant Mouse Aldehyde dehydrogenase, cytosolic 1

Protein Length

501

NCBI Gene Symbol

Aldh1a7

Protein Name

O35945

Gene Name URL

Aldh1a7

Nucleotide Accession Number

NM_011921.2

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