Aldh1a7 ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Aldehyde dehydrogenase family 1, subfamily A7
Gene Aliases
Ahd2-l; Ahd2-like; AI987940; Aldehyde dehydrogenase 1 (phenobarbitol inducible) ; aldehyde dehydrogenase family 1 member A7; aldehyde dehydrogenase family 1, subfamily A4; aldehyde dehydrogenase phenobarbital-inducible; aldehyde dehydrogenase, cytosolic 1; Aldh; ALDH class 1; Aldh1a4; ALDH-E1; Aldhpb; Aldh-pb; ALHDII.
Gene ID
26358
Accession Number
NP_036051.1
Reactivity
Mouse, Mus musculus
Target
Aldehyde dehydrogenase, cytosolic 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Aldh1a7 ELISA Kit (Mouse) (OKEH00581) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mouse Aldh1a7 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Mouse Aldh1a7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mouse Aldh1a7 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
Sensitivity
40.5 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
55 kDa
Specificity
Natural and recombinant Mouse Aldehyde dehydrogenase, cytosolic 1
Protein Length
501
NCBI Gene Symbol
Aldh1a7
Protein Name
O35945
Gene Name URL
Aldh1a7
Nucleotide Accession Number
NM_011921.2
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