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WFDC2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

WAP four-disulfide core domain 2

Gene Aliases

DJ461P17.6; EDDM4; epididymal protein 4; epididymal secretory protein E4; epididymis secretory sperm binding protein; epididymis-specific, whey-acidic protein type, four-disulfide core; HE4; human epididymis protein 4; major epididymis-specific protein E4; putative protease inhibitor WAP5; WAP domain containing protein HE4-V4; WAP four-disulfide core domain protein 2; WAP5.

Gene ID

10406

Accession Number

NP_006094.3

Reactivity

Homo sapiens, Human

Target

WAP four-disulfide core domain protein 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology WFDC2 ELISA Kit (Human) (OKEH00578) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human WFDC2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human WFDC2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human WFDC2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

13 kDa

Specificity

Natural and recombinant Human WAP four-disulfide core domain protein 2

Protein Length

124

NCBI Gene Symbol

WFDC2

Protein Name

Q14508

Gene Name URL

WFDC2

Nucleotide Accession Number

NM_006103.3

Frequently Asked Questions

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