KNG1 ELISA Kit (Bovine)

Product Specifications

CAS Number

7732-18-5

Gene Name

Kininogen 1

Gene Aliases

Bradykinin; kininogen I; kininogen II; kininogen-2; KNG; KNG2; thiol proteinase inhibitor.

Gene ID

280833

Accession Number

NP_786968.2

Reactivity

Bos taurus, Bovine

Target

Kininogen-1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology KNG1 ELISA Kit (Bovine) (OKEH00566) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Bovine KNG1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated KNG1 and incubated. The KNG1 found in the sample or standards competes with the biotinylated KNG1 for limited binding sites on the immobilized anti-Bovine KNG1 antibody. Excess unbound biotinylated KNG1 and sample or standard KNG1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated KNG1 captured in the well and inversely proportional to the amount of KNG1 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol