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MAPK3 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Mitogen activated protein kinase 3

Gene Aliases

Erk-1; ERK1; ERT2; Esrk1; extracellular signal-regulated kinase 1; insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK1; microtubule-associated protein 2 kinase; mitogen-activated 3; mitogen-activated protein kinase 1; mitogen-activated protein kinase 3; MNK1; p44; p44erk1; p44-ERK1; p44mapk; Prkm3.

Gene ID

50689

Accession Number

NP_059043.1

Reactivity

Rat, Rattus norvegicus

Target

Mitogen-activated protein kinase 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MAPK3 ELISA Kit (Rat) (OKEH00560) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rat MAPK3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Rat MAPK3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rat MAPK3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%

Sensitivity

0.09 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

43 kDa

Specificity

Natural and recombinant Rat Mitogen-activated protein kinase 3

Protein Length

380

NCBI Gene Symbol

Mapk3

Protein Name

P21708

Gene Name URL

Mapk3

Nucleotide Accession Number

NM_017347.2

Frequently Asked Questions

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