DRD1 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Dopamine receptor D1
Gene Aliases
D (1A) dopamine receptor; DADR; dopamine D1 receptor; DRD1A.
Gene ID
1812
Accession Number
NP_000785.1
Reactivity
Homo sapiens, Human
Target
D (1A) dopamine receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology DRD1 ELISA Kit (Human) (OKEH00558) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human DRD1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human DRD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human DRD1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 116%
Sensitivity
0.087 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
49 kDa
Specificity
Natural and recombinant Human D (1A) dopamine receptor
Protein Length
446
NCBI Gene Symbol
DRD1
Protein Name
P21728
Gene Name URL
DRD1
Nucleotide Accession Number
NM_000794.3
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog