CARTPT ELISA Kit (Bovine)
Product Specifications
CAS Number
7732-18-5
Gene Name
CART prepropeptide
Gene Aliases
CART; cocaine and amphetamine responsive transcript; cocaine- and amphetamine-regulated transcript protein.
Gene ID
493726
Accession Number
NP_001007821.1
Reactivity
Bos taurus, Bovine
Target
Cocaine- and amphetamine-regulated transcript protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CARTPT ELISA Kit (Bovine) (OKEH00540) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Bovine CARTPT antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CARTPT and incubated. The CARTPT found in the sample or standards competes with the biotinylated CARTPT for limited binding sites on the immobilized anti-Bovine CARTPT antibody. Excess unbound biotinylated CARTPT and sample or standard CARTPT is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CARTPT captured in the well and inversely proportional to the amount of CARTPT which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.469-30ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Sensitivity
0.226 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
13 kDa
Specificity
Natural and recombinant Bovine Cocaine- and amphetamine-regulated transcript protein
Protein Length
116
NCBI Gene Symbol
CARTPT
Protein Name
Q68RJ9
Gene Name URL
CARTPT
Nucleotide Accession Number
NM_001007820.3
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