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HSPG2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Heparan sulfate proteoglycan 2

Gene Aliases

Basement membrane-specific heparan sulfate proteoglycan core protein; endorepellin (domain V region) ; HSPG; Perlecan; perlecan proteoglycan; PLC; PRCAN; SJA; SJS; SJS1.

Gene ID

3339

Accession Number

NP_001278789.1

Reactivity

Homo sapiens, Human

Target

Basement membrane-specific heparan sulfate proteoglycan core protein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HSPG2 ELISA Kit (Human) (OKEH00535) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human HSPG2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human HSPG2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human HSPG2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.625-40ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%

Sensitivity

0.31 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

469 kDa

Specificity

Natural and recombinant Human Basement membrane-specific heparan sulfate proteoglycan core protein

Protein Length

4391

NCBI Gene Symbol

HSPG2

Protein Name

P98160

Gene Name URL

HSPG2

Nucleotide Accession Number

NM_001291860.1

Frequently Asked Questions

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